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Môi trường NBRC 979

Medium Detail Information

Medium No.

979

Medium

Clostridium thermocellum Medium (D58 Medium)

Composition

(NH₄)₂SO₄		1.3	g
MgCl₂·6H₂O		2.6	g
KH₂PO₄		1.43	g
K₂HPO₄·3H₂O		7.2	g
CaCl₂·2H₂O		0.13	g
Sodium glycerophosphate		6	g
FeSO₄·7H₂O		1.1	mg
Glutathione		0.25	g
Yeast extract		4.5	g
Resazurin		1	mg
Cellulose (Avicel or MN 300)		10	g
or Cellobiose		5	g
Distilled water		1	L
pH 7.0-7.2

	Mix ingredients except for cellobiose and autoclave under N₂/CO₂(80/20) condition. Aseptically and anaerobically add filter-sterile 10% cellobiose solution before inoculation.

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