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19 BIFIDOBACTERIUM MEDIUM
| Casein peptone, tryptic digest | 10.0 | g |
| Beef extract (BD-Difco) | 5.0 | g |
| Yeast extract (BD-Difco) | 5.0 | g |
| Glucose | 10.0 | g |
| K2HPO4 | 3.0 | g |
| Tween 80 | 1.0 | ml |
| Distilled water | 1.0 | L |
Adjust pH to 6.8.
After sterilization, aseptically add solutions of sodium ascorbate and L-cysteine·HCl to final concentration of 1.0% and 0.05%, respectively. Heat medium not freshly prepared in a steamer for 10 min before addition of the reducing substances.
